Figure 8.

Anti-Toll-like receptor 6 (TLR6) neutralizing antibody reduced interleukin-17A (IL-17A) and IL-17A-associated cytokine protein levels. At day 1 after the final Saccharopolyspora rectivirgula antigen (SR-Ag) challenge, dispersed lung cells were cultured with and without SR-Ag restimulation. The roles of TLR6 and dectin-1 were analysed by using anti-TLR6 or anti-dectin-1 neutralizing antibody. Immunoglobulin G was used as a control for these experiments. IL-17A (a), IL-6 (b), and interferion-γ (IFN-γ) (c) protein levels were measured using a multiplex bead-based assay or enzyme-linked immunosorbent assay. Data represent the mean ± SEM, with n = 3 for each group. One-way analysis of variance and Newman–Keuls multiple comparison test were used to analyse significance between groups. *P ≤ 0·05.