Figure 3.

Tumour necrosis factor-α (TNF-α) or interleukin-15 (IL-15) is not efficient to induce the expression of CD70 on monocyte-derived dendritic cells (MoDCs). Monocytes were cultured for 7 days in R10 in the presence of TNF-α or IL-15 together with granulocyte–macrophage colony-stimulating factor (GM-CSF) to induce immature TNF-DCs or IL-15-DCs, respectively. Thereafter, they were cultured for 3 days in the absence or presence of maturation-inducing stimuli (LPS or the PGE₂ cocktail). The cells were stained with fluorescein isothiocyanate (FITC) -labelled anti-CD70 or anti-CD86 monoclonal antibody (mAb). Open histograms represent cells stained with isotype-matched control mAbs. The numbers shown with each histogram represent [mean fluorescence intensity (MFI) of each costimulatory molecule]/(MFI of isotype-matched control). Data are representative of two experiments. Because there were many dead cells when TNF-DCs and IL-15-DCs were stimulated with CD40L-transfected L cells, these data are not shown. When monocytes were cultured for 3 days to induce immature DCs, similar data were obtained.