Figure 4.

Serum-free CellGro DC Medium is efficient to induce the expression of CD70 on monocyte-derived dendritic cells (MoDCs). Monocytes were cultured for 3 days in R10 or CellGro DC Medium (CG) in the presence of interleukin-4 (IL-4) or interferon-α (IFN-α) together with granulocyte–macrophage colony-stimulating factor (GM-CSF) to induce immature DCs. Thereafter, they were stimulated with CD40L-L cells or the prostaglandin E₂ (PGE₂) cocktail for 3 days in the same medium used for the first 3 days. The cells were stained with fluorescein isothiocyanate (FITC) -labelled anti-CD70 or anti-CD86 monoclonal antibody. Data are shown by [mean fluorescence intensity (MFI) of CD70]/(MFI of isotype-matched control). Data are representative of three experiments.