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Generation of a TIMP-3–expressing oHSV. A, genomic map of parental virus, rHSVQ1, and new viruses rQLuc and rQT3. B, assessment of hTIMP-3 expression, via intracellular flow cytometry, in LA-N-5 cells infected with oHSV for 5 h. C, luciferase assay on protein lysates from rQLuc-infected STS26T, ST8814, S462, and Vero cells infected with 1 pfu/cell.
