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. 2010 Apr;15(4):397–407. doi: 10.1111/j.1365-2443.2010.01388.x

Figure 1.

Figure 1

The nucleus of posterior daughter cell is anchored to the cell cortex by centrosomes after an asymmetric division. (a, b) Time-lapse images of the same embryos expressing GFP::β-tubulin (a) or GFP::γ-tubulin (b) in the focal planes in which the center of anterior or posterior centrosomes was clearly observed as indicated in the top of the panels. The numbers on the left indicate the amount of time (minutes : seconds) after furrowing onset. Asterisks indicate the center of the anterior or posterior nuclei. Arrowheads indicate the positions of centrosomes. Both centrosomes collapsed and duplicated around 4:00. Some of the duplicated centrosomes were out of focus in the images after 5:20. In (b), the boundaries of the MS and E cells are outlined by dotted lines in the left and center panels, respectively. The right panels show the magnified images of the middle panels around the posterior side of the E cell with its boundary to the P2 cell outlined by dotted lines. (c) Merged differential interference contrast (DIC) and GFP images of wild-type or zyg-12(ts) embryos expressing GFP::γ-tubulin at 6 min after furrowing onset. The right panels show the magnified images of the left panels around the posterior side of the E cell with their boundaries to the P2 outlined by dotted lines. Arrowheads and asterisks indicate the positions of centrosomes and center of the nuclei in the E cell, respectively. Bars, 5 μm.