Figure 2.

The nuclear anchoring in various cells. (a) The positions of nuclei and centrosomes in indicated embryonic daughter cells were observed at 320 s after furrowing onset (approximately 2.5 min after the completion of the divisions) of their mother cells (See also Fig. S1 in Supporting Information). For postembryonic T and V5 cells, nuclei were observed at late telophase or just after the division. In each daughter cell pair, the upper and lower rows in the table indicate anterior and posterior daughter cells, respectively. Cen0, Cen1 and Cen2 indicate that zero, one and two centrosomes, respectively, were observed to be attached to the cortex as schematically described at the bottom of the table. In all cases, centrosomes were attached to nuclei. Nuclei were anchored to the cortex in Cen1 and Cen2. (b) Left and right are images of the same embryos in the focal planes with focuses on the anterior and posterior daughter cells, respectively. Cell boundaries are outlined by dotted lines. Asterisks and arrowheads indicate the center of the nuclei and the positions of centrosomes, respectively. Bars, 5 μm.