Table 1.
Behavioral assays within each phenotyping battery
| Sample sizes within strain |
||||||
|---|---|---|---|---|---|---|
| Battery (site) | Test description | Apparatus (Mfg, model, dimensions) | Test order | Average | Max | Number of strains |
| Adrenals (UTHSC) | Left and right adrenal weights | Zeiss Dissecting Microscope, Mettler Toledo scale, Bouin's Fixative, Paraplast Plus Tissue Embedding Medium (McCormick Scientific, St. Louis, MO, USA) | Single measure | 4♂, 4♀ | 8♂, 8♀ | 62♂, 63♀ |
| Adult neurogenesis (UTHSC) | Number of BrdU+ cells per millimeter length in the RMS | Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) AnalySIS Opti Version 3.3.776 | Single measure | 3♂, 3♀ | 5♂, 5♀ | 40♂, 36♀ |
| Cocaine (U. Memphis) | Cocaine-conditioned place preference; locomotor activity in a novel environment, after saline, and after each of two cocaine treatments | Eight Med-Associates automated open field chambers (43.2 cm L × 43.2 cm W × 30.4 cm H; part no. ENV-515), Eight Med-Associates place preference chambers (46 cm L × 14 cm W × 20 cm H; part no. ENV-3013) | Fixed: OF-novel, OF-saline, OF-cocaine, OF-cocaine 2, cocaine place preference; morphine withdrawal (naïve set) | 11♂, 10♀ | 21♂, 28♀ | 66♂, 64♀ |
| Ethanol 1 (UTHSC) | OFA after saline and ethanol; elevated plus maze after saline and ethanol; rotarod ataxia | Columbus Instruments Rotarod and Plus Maze, AccuScan Activity Chamber, Razor blade, Analox tube, Centrifuge | Ethanol first in half of mice, saline first in half of mice, fixed: OFA, EPM, rotarod | 4♂, 3♀ | 6♂, 6♀ | 56♂, 48♀ |
| General behavior (U. Memphis) | Hot plate; light–dark box, zero-maze; open field activity; pre-pulse inhibition; acoustic startle response | AccuScan Instruments Elevated Zero-Maze, Hamilton-Kinder Smart Frame Monitor with Open Field and Light/Dark Insert, IITC Hot-Plate Unit, Hamilton-Kinder SM100 Startle Monitors | Fixed: Hot plate, light-dark box, zero-maze, open field activity, acoustic startle response, pre-pulse inhibition | 10♂, 9♀ | 29♂, 32♀ | 60♂, 58♀ |
| MDMA (Meharry) | Open field activity in a novel environment; locomotor activity after saline or MDMA | Med Associates Open Field Activity System MED-OFA-510, Digital video camera | Fixed: OF, FST | 2♂, 2♀ | 4♂, 2♀ | 20♂, 15♀ |
| Alcohol withdrawal (U. Memphis) | HICs–baseline and at 1 h intervals after EtOH | N/A | Fixed: baseline, post-injection intervals | 5♂, 5♀ | 14♂, 12♀ | 36♂, 30♀ |
| Morphine (U of Memphis) | Open field activity in a novel environment and after morphine, salivation, defecation, urination, postural effects, wet dog shakes and ptosis after morphine treatment | Eight Med-Associates automated open field chambers (43.2 cm × 43.2 cm; part no. ENV-515) | Fixed: OF after saline, OF after morphine | 6♂, 6♀ | 21♂, 28♀ | 62♂, 62♀ |
| Pain (ORNL) | Hargreaves' test, hot plate, tail withdrawal, tail clip, von Frey test | IITC Life Science Inc., 336 TG Plantar Test (Hargreaves) and Tail Flick Analgesia Meter with Heated Glass and Tail Temperature, IITC Life Science Inc., Hot-Plate Analgesia Meter, Boekel Scientific/Grant Optima Immersion Circulator Model GR150, 600g Alligator Clamp w/Heat-Shrink coating, von Frey Fiber Set Stoelting Inc. | Unique Latin Square within each strain and sex | 7♂, 6♀ | 10♂, 10♀ | 60♂, 54♀ |
| Ethanol 2 (UTHSC) | Dowel test, Porsolt forced swim test | Piezo System, Swimming Chamber, Dowel, LX-101 Lux meter | Fixed: sleep, Porsolt, Dowel | 4♂, 4♀ | 11♂, 7♀ | 55♂, 47♀ |
| Vocalization (U of Memphis) | Footshock vocalization threshold | Med Associates Inc., Shock Titration Package for Mice (model ENV-307 W) | Single measure, repeated shock with increasing mA intensity | 5♂, 5♀ | 9♂, 8♀ | 67♂, 66♀ |