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. Author manuscript; available in PMC: 2010 Apr 18.
Published in final edited form as: Methods Mol Biol. 2008;442:11–27. doi: 10.1007/978-1-59745-191-8_2

Fig. 1.

Fig. 1

Recombinant Dicer-1 bacmids contain full-length Dicer-1 cDNA. The 5′ and 3′ PCR reactions were performed without DNA template (lane 1 and 2) or with 1 μL of recombinant Dicer-1 bacmids prepared from four positive transformants (lanes 3–10). The 5′ PCR reactions use a 5′ bacmid (forward) primer (5′-GTTTTCCCAGTCACGAC-3′) and a 5′ Dicer-1 (reverse) primer (5′-CCGTCCAGCAATGATCAAAG-3′). The 3′ PCR reactions use a 3′ Dicer-1 (forward) primer (5′-TTCCACAAGTTCTTCCGGCA-3′) and a 3′ bacmid (reverse) primer (5′-CAGGAAACAGCTATGAC-3′). The predicted size of 5′ and 3′ PCR products are ∼ 2 kb and 1 kb.