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. Author manuscript; available in PMC: 2010 Apr 18.
Published in final edited form as: Methods Mol Biol. 2008;442:11–27. doi: 10.1007/978-1-59745-191-8_2

Fig. 3.

Fig. 3

Purification of recombinant Dicer-1 and Dicer-2. The His-tagged Dicer-1 and Dicer-2 proteins are both purified by Ni+2-affinity purification followed by SP-Sepharose and Q-Sepharose chromatography. (a) Lane 1: 250 mM imidazole elution, also as input of SP column; lane 2: SP flowthrough, also as input of Q column; lanes 3–7: Dicer-1 peak fractions of Q column. (b) Lane 1: 250 mM imidazole elution, also as input of SP column; lane 2: Dicer-2 peak fractions of SP column; also combined as input of Q column; lane 3: Dicer-2 peak fractions of Q column.

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