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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Neuroscience. 2009 Mar 24;161(1):227–235. doi: 10.1016/j.neuroscience.2009.03.044

Figure 1.

Figure 1

A–F. Examples of 7-day, confluent cultures immunolabeled as shown in italics. G–L. Examples of 42 hour cultures maintained under different growth factor and substrate conditions and immunolabeled with TuJ1 to highlight neurons. (A). The nerve fiber pattern in this longer term culture is non-uniform. Fibers extend in some regions and avoid others (asterisk), growing singly (arrowhead) but also, often growing in bundles (double arrowhead) atop the underlying non-neural cells. (B–D) Endogenously generated laminin-1 (B), entactin (C) or integrin β4 (D) form filamentous, non-uniform patterns in the culture; (E) Fibronectin is expressed fairly homogenously across the nonneural cells. (F) Tenascin immunoreactivity is observed in islands of stain across the culture well. (G–L) Under all tested conditions (control, LIF or BMP4 medium and laminin, fibronectin or tenascin coating of the culture well), neurites grow only in association with a type of non-neural cell that is usually spindle shaped (arrows).Neurites and spindle-shaped cells retain their associations even throughout abrupt changes in the direction of growth (see K).