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. Author manuscript; available in PMC: 2010 Apr 18.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):329–339. doi: 10.4049/jimmunol.182.1.329

FIGURE 8.

FIGURE 8

The Btk xid mutation decreases the rate of BCR internalization in both mature and transitional splenic B cells. Splenic B cells from wt and xid mice were labeled with PE-Cy5 anti-mouse B220, FITC-anti-mouse AA4.1 (CD93), and biotin-F(ab′)2-anti-mouse IgM at 4°C and chased at 37°C for varying lengths of time. BCR internalization was analyzed as described in Fig. 7. BCR internalization in mature and immature B cell subsets (B) was measured by gating for B220+AA4.1− (mature) and B220+AA4.1+ (immature/transitional B cells) (A). Shown are data from two independent experiments.