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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: J Immunol Methods. 2010 Feb 8;355(1-2):86–90. doi: 10.1016/j.jim.2010.01.013

Figure 1.

Figure 1

OVA expression in transgenic T. gondii. Intracellular parasites were isolated from HFFs by forced rupture using a 27 gauge needle. Parasite protein was prepared as described in methods. 50 μg of T. gondii protein was separated by SDS-PAGE. T. gondii β-tubulin was used for normalization. Ova protein is detected in Me49-Ova strain just after purification and remains at the same level after 6 months of passage in HFFs. No Ova protein is detected in parental Me49.