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. 2010 Mar 23;102(8):1224–1234. doi: 10.1038/sj.bjc.6605617

Figure 7.

Figure 7

Induction of apoptosis by α-tocopheryl succinate (α-TOS) or vitamin K3 (VK3) plus ascorbic acid (AA) alone or in combination in the presence of a lysosomal protease inhibitor. PC3 cells were exposed for 24 h to sub-apoptotic doses of α-TOS (30 μM) or VK3 (3 μM) plus AA (0.4 mM) alone or in combination, and (A) lysosmal and (B) mitochondrial destabilisation were evaluated using the Acridine orange (AO) and MitoTracker Red-580 staining, respectively, in the presence or absence of the lysosomal protease inhibitor E-64d. (C) Cytochrome c release and cell viability after treatments of prostate cancer cells as described above in the presence or absence of lysosomal protease inhibitor E-64d was assessed. The sign ‘*’ denotes significantly different data for treated and control cells with P<0.05.