Figure 2.
10% SDS-PAGE analysis of mutant procapsids. Lane 1, N-Ex procapsid, molecular weight (MW) of N-Ex gp10 was 40.2 kDa; lane 2, N-Ex procapsid cleaved by TEV. MW of treated N-Ex gp10 was 35.9 kDa; lane3, C-Ex procapsid. MW of C-Ex gp10 was 37.3 kDa; lane 4, N-DeEx procapsid. MW of N-DeEx gp10 was 36.8 kDa; lane 5, N-DeEx procapsid cleaved by TEV. MW of treated N-DeEx gp10 was 35.9 kDa; lane 6, C-DeEx procapsid. MW of C-DeEx gp10 was 34.3 kDa; lane 7, normal procapsid. MW of normal gp10 was 35.9 kDa. The small arrowhead refers to the mutated gp10, which has been treated by TEV, removing the N-terminus peptide tag. The star refers to the TEV protease.