Table 2.
Sequence of the PCR primers used for the plasmid construction of mutant procapsids*
Procapsid | Primers | Sequence (5′–3′) |
---|---|---|
Procapsid N-Ex | F1 | TAATGTGGAGCCACCCGCAGTTCGAAAAGGGAGGTGGTGGAGGAGGTGGAGGTGGTGGAGC |
R1 | TACACCTCGGTGGGCGTCAAGCTTTTCCCTCCACCACCTCCTCCACCTCCACCACCTCGAT | |
Procapsid C-Ex | F1, F2 | CGCAGCTGGCATATGGCACGTAAACGCAGTAAC |
R1 | GGATGACTCCAACCTCCTCCACCACCTCCCTCATTTGTTTCACCGT | |
R2 | ATAATGTTCTCGAGCTACTTTTCGAACTGCGGATGACTCCAACCTC | |
Procapsid N-DeEx | F1 | GTAACCTGCATATGCACCATCACCATCACCATGATTACGATATCCCAACGACTGA |
F2 | CGATATCCCAACGACTGAAAACCTGTACTTCCAGGGAATACAGCGTCAAAAACGG | |
R1, R2 | CTAGCTATCTCGAGTTAGATGTCATATCTGAATTTAACTT | |
Procapsid C-DeEx | F1, F2 | CGCAGCTGGCATATGGCACGTAAACGCAGTAAC |
R1 | GGATGACTCCAACCTCCTCCACCACCTCCGATGTCATATCTGAATT | |
R2 | ATAATGTTCTCGAGCTACTTTTCGAACTGCGGATGACTCCAACCTC |
F1/R1 and F2/R2 were the first and second pair of primers, respectively.