Fig. 1.
Embryonic expression patterns of Scr in Periplaneta americana. (A) At ≈10% development, Scr mRNA is broadly expressed in the mid-posterior region with two strong bands that correspond to the future Lb/T1 region. (B) At ≈20% development, strong Scr mRNA localizes to the labial segment. (C) Slightly later at ≈25%, Scr continues to be solely expressed in the labium with no signal in the Mx or T1 segments. (D) SCR protein accumulation in an embryo at ≈75% development. While strong expression remains in the labial palps, SCR has now expanded into the T1 segment with clear signal in the dorsal T1 region (orange arrowhead) and in two discrete clusters of cells in the T1 leg (white arrowheads). (E) RNAiScr embryo that has been stained for Scr mRNA accumulation. The lack of signal indicates the complete depletion of Scr.
Abbreviations: Mn = Mandibles, Mx = Maxillary, Lb =Labial, T1 = First thoracic segment
