Figure 5.

Lag phase, seeded growth, and nature of the nucleus. (A) Dependence of kinetics on initial peptide concentration at 65 μM (+), 70 μM (×), 80 μM (^∗), 90 μM (□), 100 μM (■), 120 μM (○), and 140 μM (•) in each peptide. The gray line shows a model fit for the early time points. (B) Fraction completion as a function of time for a seeded sample 80 μM (+); the fit (solid line) is to a single exponential. (C) Fiber growth rate after seeding as a function of initial concentration of peptide. (D) Lag time as a function of the concentration of partially helical dimers (determined from Fig. 3B).