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Manipulating SAF length by seeding. Fibers were grown for 1 h from 60 μM samples with (A) 10%, (B) 5%, (C) 2%, and (D) 1% (v/v) of matured fibers from standard 100 μM preparations fragmented after 24 h. Scale bar: 10 μm. (E) Histograms showing the number of fibers as a function of length for the 10% (black) and 1% (gray) seeded samples. (F) Cumulative frequencies for 10% (solid black), 5% (dashed), 2% (dot-dashed), and 1% (gray) seeded samples. Number of observations: 250–1500.
