FIGURE 1.

A PI3-K-specific inhibitor reduces the RA-induced secretion of sAPPα, whereas the sAPLP2 levels are not significantly changed. Relative abundance of sAPPα (A) and sAPLP2 (B) in culture medium, normalized to the relative abundance of full-length APP or APLP2 in cell lysates from SH-SY5Y cells treated with 1 μm RA for 6 days in the absence or presence of 10 μm LY 292002 (LY) for the last 18 h. Data represent mean ± S.E., n = 4–7. **, p < 0.01 significantly different from cells treated with RA. Representative Western blot analyses of secreted and full-length APP and APLP2 are shown below the graphs. Untreated control (C) cells are included for comparison. The vertical lines indicate the quantified bands.