FIGURE 2.

Identification of mvs mutants that suppress the poor growth phenotype of Δmrs3Δmrs4 cells overexpressing Ccc1. A, wild type (WT), Δmrs3Δmrs4, mvs6-3, and mvs6-4 cells were transformed with a MET3CCC1 plasmid, grown overnight in selection media, and washed, and serial dilutions were spotted on plates with (CCC1 off) or without 10× methionine (CCC1 on) and grown for 2 days at 30 °C. B, MVS mutant 6-3 and 6-4, cured of the MET3CCC1 plasmid, were spotted on plates containing the specified amounts of cobalt or copper and grown for 2 days at 30 °C. All experiments were performed a minimum of three times.