FIGURE 3.

Identification of RIM2 and FRA1 as suppressors of the MVS phenotype. A, wild type (WT) or Δmrs3Δmrs4 cells were transformed with a control plasmid pFRA1, or pRIM2 under the control of their endogenous promoters. The cells were also transformed with pMET3CCC1, serial diluted onto plates with (CCC1 off) or without 10× methionine (CCC1 on) and grown for 2 days at 30 °C. B, wild type or Δmrs3Δmrs4 cells transformed with a control plasmid, pRIM2, or pFRA1 under the control of their endogenous promoters were grown for 2 days at 30 °C on low iron (BPS2) plates. C, wild type, Δmrs3Δmrs4, and Δmrs3Δmrs4 with pFRA1 were grown for 2 days on CM plates supplemented with 4 mm Co²⁺, 1.5 mm Cu²⁺, and 25 μm Cd²⁺. All experiments were performed a minimum of three times.