FIGURE 5.

FRA2 is not involved in the MVS phenotype. A, Δmrs3Δmrs4 cells were transformed with empty vector, pFRA1 was transformed with endogenous promoter, pFRA1 was transformed with a GAL1 promoter, or pFRA2 was transformed with a GAL1 promoter followed by transformation with pMET3CCC1. Cells were washed and spotted in serial dilution on galactose plates with (CCC1 off) or without 10× methionine (CCC1 on) and grown for 3 days at 30 °C. Galactose was used as a carbon source to turn on GAL1-regulated genes. B, wild type (WT), Δmrs3Δmrs4, and Δmrs3Δmrs4Δfra2 cells were serial-diluted on YPD or CM plates and grown for 2 days at 30 °C. All experiments were performed a minimum of three times.