TABLE 1.
Ligand binding properties of wild type GABABR and three combinations of intersubunit FRET pairs
Kd and Bmax values for each combination were derived from the saturation binding assay for radiolabeled antagonist [3H]CGP54626 as described under “Experimental Procedures.” The values are means ± S.E. of three separate assays.
| Kd | Bmax | |
|---|---|---|
| nm | pmol/mg | |
| Wild type | 20.4 ± 3.7 | 1.80 ± 0.23 |
| GB1a-i2 and GB2-i1 | 69.8 ± 11.1 | 1.66 ± 0.05 |
| GB1a-i1 and GB2-i2 | 28.7 ± 10.3 | 0.281 ± 0.108 |
| GB1a-i3 and GB2-i3 | 16.2 ± 2.2 | 1.08 ± 0.10 |