FIGURE 1.

ELG1 interacts with USP1-UAF1 complex. A, the USP1 complex was purified from HeLa nuclear extracts and stained by Coomassie blue stain. Proteins indicated were identified by mass spectrometry. B, nuclear extracts from HeLa cells were immunoprecipitated (IP) with anti-USP1-antibody or IgG control antibody. The immunoprecipitated proteins were identified with the antibodies indicated. C, HEK293T cells were transected with FLAG-ELG1 expression vectors or control vectors (Ctrl). Total extracts from the transfected cells were IP after 48 h with anti-FLAG-antibody. The immunoprecipitated proteins were identified with the antibodies indicated.