FIGURE 3.
DEX reduces myogenic differentiation and up-regulates GILZ and L-GILZ expression in C2C12 myoblast cultures. A, C2C12 myoblasts were induced to differentiate for 48 h in absence (CNTRL, panel a) or in presence (panel b) of 10−5 m DEX. The cultures were subjected to MyHC staining. Bar, 50 μm. B, Western blot analyses of GILZ/L-GILZ induction by DEX and of expression of biochemical markers of differentiation in CNTRL and DEX-treated cells at the indicated time points. C, densitometric analysis of the Western blots from B are shown in C (myogenin/β-tubulin ratio).