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Effect of ionic strength on reverse MDH activity enhancement by bc1. MDH only or together with bc1 complex in 100 mm Na+/K+ phosphate buffer, pH 7.5, was incubated with the indicated concentrations of NaCl on ice for 30 min before MDH activity (the reverse reaction) was determined. The activity of MDH only was used as 100%. n = 4, data are the means ± S.D.
