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GLP-1 and IBMX differentially increase cAMP levels in MIN6 cells. MIN6 cells were incubated in the presence or absence of GLP-1 (100 nm) or IBMX (10 μm) for 30 s or 2 min, and intracellular cAMP contents were measured by radioimmunoassay. The data are the means ± S.D., n = 3 independent experiments. **, p < 0.01 (treatments compared with control); §§, p < 0.01 (IBMX treatment compared with GLP-1 treatment).
