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. 2010 Feb 4;285(14):10572–10582. doi: 10.1074/jbc.M109.050534

TABLE 1.

Gene-specific primer pairs used in real-time quantitative PCR

Gene Sense Antisense Amplicon length (pb) GenBankTM accession number
Aggrecan 5′-ACA CCC CTA CCC TTG CTT CT-3′ 5′-AAA GTG TCC AAG GCA TCC AC-3′ 124 NM_022190
Ank 5′-CAA GAG AGA CAG GGC CAA AG-3′ 5′-AAG GCA GCG AGA TAC AGG AA-3′ 173 NM_053714
PC-1 5′-TAT GCC CAA GAA AGG AAT GG-3′ 5′-GCA GCT GGT AAG CAC AAT GA-3′ 165 NM_053535
Runx-2 5′-TAT TCC CGT AGA TCC GAG CA-3′ 5′-GCT CAC GTC GCT CAT CTT G-3′ 82 NM_053470
S29 5′-AAG ATG GGT CAC CAG CAG CTC TAC TG-3′ 5′-AGA CGC GGC AAG AGC GAG AA-3′ 67 NM_012876
Sox-9 5′-CTG AAG AAG GAG AGC GAG GA-3′ 5′-GGT CCA GTC ATA GCC CTT CA-3′ 84 AB 073720
TNAP 5′-GAA CGT CAA TTA ACG GCT GA-3′ 5′-CAG ATG GGT GGG AAG AGG T-3′ 50 NM_013059
Type IA2 collagen 5′-TTG ACC CTA ACC AAG GAT GC-3′ 5′-CAC CCC TTC TGC GTT GTA TT-3′ 197 NM_053356
Type II collagen 5′-TCC CTC TGG TTC TGA TGG TC-3′ 5′-CTC TGT CTC CAG ATG CAC CA-3′ 161 NM_012929
Type X collagen 5′-ATA TCC TGG GGA TCC AGG TC-3′ 5′-TGG GTC ACC CTT AGA TCC AG-3′ 241 AJ131848
Wnt-3a 5′-ACT GCA CCA CTG TCA GCA AC-3′ 5′-CAT GGA CAA AGG CTG ACT CC-3′ 81 NM_001107005
Wnt-5a 5′-AAA TAG GCA GCC GAG AGA CA-3′ 5′-GGC TCA TGG CAT TTA CCA CT-3′ 69 AB 244721
Wnt-5b 5′-AAC GTG GAG TAT GGC TAC CG-3′ 5′-TGA TCC CTT GGC AAA GTT CT-3′ 78 NM_001100489
Wnt-7a 5′-CAC AAT TCC GAG AGC TAG GC-3′ 5′-ACG GCC TCG TTG TAT TTG TC-3′ 52 NM_009527
Wnt-11 5′-CTG ACC TCA AGA CCC GCT AC-3′ 5′-TAG GCC GGT GTA CCA CTT TC-3′ 51 NM_080401