FIGURE 2.
ApoB100-containing lipoproteins are produced by mouse kidney. A, laser confocal microscopy (combined fluorescent and differential interference contrast micrograph) showing human apoB protein staining (red) in kidney tubular epithelial cells in a human apoB-transgenic mouse. B, control-stained section without primary antibody. C, kidney tissue from a human apo-B transgenic mouse was incubated with 35S-labeled amino acids. The medium was subsequently subjected to sucrose density gradient ultracentrifugation. ApoB was isolated from each density fraction by immunoprecipitation and analyzed by SDS-PAGE and filmless autoradiographic analysis. 35S-ApoB100 was seen in the d ∼1.03–1.04 g/ml lipoprotein fractions.