FIGURE 1.

Loss of SOC in Wilms tumor cells. A, SOCE was measured in Fura-2-loaded WT10, WT11, and HEK293 cells after ER Ca²⁺ depletion via the addition of the SERCA inhibitor thapsigargin (Tg; 2 μm) in nominally Ca²⁺-free medium. Extracellular Ca²⁺ concentration was increased from 0 to 1 mm either before or after store depletion where indicated to differentiate between store-independent (before thapsigargin) and store-dependent (after thapsigargin) Ca²⁺ entry. Shaded areas indicate S.E. B, protein extracted from WT10, WT11, and HEK293 cells was analyzed by Western blotting for STIM1 expression. actin was used as a loading control. C, RNA extracted from WT10, WT11, and HEK293 cells was analyzed by quantitative PCR for expression of TATA-binding protein (TBP), STIM1, WT1, and EGR1. mRNA expression levels are shown as %TBP. *, significant differences from expression levels in HEK293 cells as determined by analysis of variance with Tukey's post-hoc test (p < 0.05). All experiments were completed a minimum of three times.