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. 2010 Feb 1;285(14):10616–10626. doi: 10.1074/jbc.M109.069070

FIGURE 2.

FIGURE 2.

Inhibition of auto-aggregation by purified AIDA-I and not by cellular extracts. A, cultures bearing an empty vector (-) or expressing AIDA-I (WT) were incubated in deep well 96-wells plates with or without total lysates (L) or membrane extracts (M) prepared from cultures harboring the empty vector or expressing AIDA-I and left standing for 90 min. The columns were compared with the wild-type control by performing a one-way ANOVA and Dunnett's post tests. *, statistical significance (p < 0.05); NS, not significant. B, auto-aggregation assays of cultures of bacteria expressing AIDA-I were performed with various concentrations of purified AIDA-I present. The points were fitted by nonlinear regression to a sigmoidal dose-response. C, normalized cultures of bacteria harboring an empty vector or expressing AIDA-I were incubated with or without 200 nm wild-type protein, mutated protein I24, or heat-extracted purified protein (HE). The columns were compared with the wild-type vector control by performing a one-way ANOVA and Dunnett's post tests. *, statistical significance (p < 0.05); NS, not significant.