FIGURE 4.

Age of htt-98Q mice influences accumulation of soluble or aggregated transgenic htt in astrocytes. A, double immunofluorescent staining of mouse brain sections containing the Str and WM from wild-type, htt-98Q, and N171–82Q mice. The staining was performed with mouse 1C2 antibody to htt (red) and rabbit antibody to GFAP (green). Note that mutant htt staining is enriched in GFAP-positive cells in the WM of htt-98Q mice or in neuronal cells of N171–82Q mice. Merged images also show nuclear staining (blue). B, age-dependent accumulation of mutant htt in astrocytes of htt-98Q transgenic mice. Note that mutant htt is diffuse or forms small aggregates in the cytoplasm of astrocytes in young (3 and 9 month) mouse brain and can form nuclear inclusions (arrow) in old (16 month) mouse brain. Scale bars: 10 μm.