TABLE 2.
Constitutive gene expression and higher oleate induction with Adr1c and Adr1 overexpression
Values are average and standard deviation of two biological replicates expressed as fold overexpression with wtAdr1.
| Adr1a/conditionb | ADH2 | CTA1 | FAA2 | FOX2 | POT1 | POX1 | SPS19 |
|---|---|---|---|---|---|---|---|
| Adr1c R | 110 ± 1.8 | 19 ± 7.3 | 17 ± 0.66 | 21 ± 5.1 | 28 ± 1.2 | 53 ± 4.7 | 26 ± 7.3 |
| Adr1c DRI | 4.8 ± 0.45 | 6.6 ± 4.9 | 3.0 ± 1.6 | 3.5 ± 1.9 | 2.0 ± 0.69 | 6.7 ± 1.8 | 3.2 ± 1.9 |
| 2-μm Adr1 R | 390 ± 37 | 8.6 ± 3.4 | 6.0 ± 0.23 | 4.6 ± 1.5 | 6.0 ± 0.59 | 32 ± 8.2 | 8.1 ± 2.4 |
| 2-μm Adr1 DRI | 33 ± 3.9 | 16 ± 9.4 | 8.4 ± 1.6 | 9.0 ± 2.9 | 4.5 ± 1.4 | 16 ± 8.8 | 13 ± 3.6 |
a CEN plasmids expressing Adr1 (pKD16), Adr1c (pKD14), or 2-μm plasmid expressing Adr1 (pKD17) were transformed in CKY13.
b Repression (R) was in 5% glucose for 18 h; oleate induction (derepressed-induced, DRI) was for 1 h in 0.1% oleate + 3% glycerol.