TABLE 3.

Oleate induction of peroxisomal genes occurs in glucose in the presence of Adr1^c

A. Oleate induction occurs in repressing conditions in the presence of Adr1c
Adr1a	CTA1	FOX2	POT1	POX1
Wild type	8.0 ± 0.09	7.0 ± 0.2	2.0 ± 0.02	8.0 ± 0.03
Adr1c	99 ± 15	100 ± 43	82 ± 6	61 ± 27

B. Adr1c-dependent oleate induction in repressing conditions occurs in the absence of Snf1 but is dependent on Oaf1/Pip2
Genotypeb	CTA1	FOX2	POT1	POX1
snf1Δ	38 ± 0.04	71 ± 14	79 ± 0.08	66 ± 7
oaf1Δ	26 ± 5.0	52 ± 7.0	5 ± 0.8	5 ± 0.2
pip2Δ	37 ± 0.03	68 ± 9.0	9.0 ± 0.6	10 ± 2

^a CEN-TRP1 plasmids expressing wild type Adr1 (pKD16) or Adr1^c (pKD14; S230A) were transformed in CKY13 (adr1Δ). Gene expression was measured by qPCR 8 h after addition of 0.1% oleate to glucose-repressed cultures. The data are presented as the percent of expression with Adr1^c in glucose-derepressing, oleate-inducing conditions; errors indicate standard deviation (as percent) of three biological samples.

^b The adr1Δ strains employed are listed in Table 1. Gene expression in strains carrying Adr1^c on pKD14 with deletion of SNF1, OAF1, or PIP2 was assayed by qPCR in glucose-repressed cultures 8 h after 0.1% oleate induction (repressing). Values are expressed as percent of expression in the same conditions, but with WT SNF1, OAF1, and PIP2; errors indicate standard deviation (as percent) of three biological samples.