TABLE 3.
A. Oleate induction occurs in repressing conditions in the presence of Adr1c | ||||
---|---|---|---|---|
Adr1a | CTA1 | FOX2 | POT1 | POX1 |
Wild type | 8.0 ± 0.09 | 7.0 ± 0.2 | 2.0 ± 0.02 | 8.0 ± 0.03 |
Adr1c | 99 ± 15 | 100 ± 43 | 82 ± 6 | 61 ± 27 |
B. Adr1c-dependent oleate induction in repressing conditions occurs in the absence of Snf1 but is dependent on Oaf1/Pip2 | ||||
---|---|---|---|---|
Genotypeb | CTA1 | FOX2 | POT1 | POX1 |
snf1Δ | 38 ± 0.04 | 71 ± 14 | 79 ± 0.08 | 66 ± 7 |
oaf1Δ | 26 ± 5.0 | 52 ± 7.0 | 5 ± 0.8 | 5 ± 0.2 |
pip2Δ | 37 ± 0.03 | 68 ± 9.0 | 9.0 ± 0.6 | 10 ± 2 |
a CEN-TRP1 plasmids expressing wild type Adr1 (pKD16) or Adr1c (pKD14; S230A) were transformed in CKY13 (adr1Δ). Gene expression was measured by qPCR 8 h after addition of 0.1% oleate to glucose-repressed cultures. The data are presented as the percent of expression with Adr1c in glucose-derepressing, oleate-inducing conditions; errors indicate standard deviation (as percent) of three biological samples.
b The adr1Δ strains employed are listed in Table 1. Gene expression in strains carrying Adr1c on pKD14 with deletion of SNF1, OAF1, or PIP2 was assayed by qPCR in glucose-repressed cultures 8 h after 0.1% oleate induction (repressing). Values are expressed as percent of expression in the same conditions, but with WT SNF1, OAF1, and PIP2; errors indicate standard deviation (as percent) of three biological samples.