TABLE 1.

IC₅₀ values and relative activities of lysine mutants against H. contortus and T. colubriformis

	Mutant	H. contortus		T. colubriformis
		IC50	Relative activitya	IC50	Relative activitya
		μm		μm
Wild-type	kB1	2.7	1.0	4.5	1.0
Loop 1	G6K	>11.5	<0.2	>11.5	<0.4
	E7K	>11.5	<0.2	>11.5	<0.4
	T8K	>11.5	<0.2	>11.5	<0.4
Loop 2	V10K	>11.5	<0.2	>11.5	<0.4
	G11K	5.3	0.5	6.1	0.7
	G12K	>11.5	<0.2	>11.5	<0.4
	T13K	2.8	1.0	4.0	1.1
Loop 3	N15K	>11.5	<0.2	>11.5	<0.4
	T16K	>11.5	<0.2	>11.5	<0.4
	P17K	3.6	0.7	4.9	0.9
	G18K	1.1	2.4	1.8	2.5
Loop 4	T20K	0.9	3.0	1.7	2.6
Loop 5	S22K	2.3	1.1	2.7	1.7
	W23K	>11.5	<0.2	>11.5	<0.4
	P24K	N/Db	N/D	N/D	N/D
	V25K	>11.5	<0.2	>11.5	<0.4
Loop 6	T27K	1.6	1.7	3.4	1.3
	R28K	3.9	0.7	7.7	0.6
	N29K	0.4	7.0	1.2	3.8
	G1K	0.5	5.8	1.5	2.9
	L2K	6.2	0.4	6.6	0.7
	P3K	>11.5	<0.2	>11.5	<0.4
	V4K	11.1	0.2	>11.5	<0.4
Double-lysine	T20K/S22K	0.7	4.1	1.0	4.5
	T20K/N29K	0.4	6.5	0.7	6.8
	T20K/G1K	0.4	6.5	0.7	6.8
Triple-lysine	T20K/N29K/G1K	0.2	13.0	0.7	6.2

^a Expressed relative to wild-type kalata B1.

^b N/D, not determined.