FIGURE 5.

Suppression of chemotactic migration of B16F10 cells by blocking antibody to RAGE. B16F10 cells were preincubated with a blocking goat antibody (Ab) to RAGE or with a nonspecific goat IgG for 1 h prior to performing the in vitro migration assay using medium containing either S100A8 or S100A9 or a combination of S100A8 and S100A9 as chemoattractants. Medium containing no chemoattractants served as control. The results showed that treatment of the cells with the blocking antibody to RAGE significantly suppressed the migration of the B16F10 cells. Representative photomicrographs for each treatment are shown in A. These results were further confirmed by quantitation of the migrating cells under the microscope (B).