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. 2010 Jan 29;285(14):10822–10831. doi: 10.1074/jbc.M109.083550

FIGURE 6.

FIGURE 6.

Stimulation of MMP expression in B16F10 cells by S100A8 and S100A9. A, the levels of MMP-2, MMP-9, MMP-14, and furin mRNAs were significantly up-regulated upon treatment with S100A8 and S100A9 at a concentration of 1 μg/ml for 24 h. Cells without any treatment were used as control. Consistent with the mRNA levels, the protein levels of MMP-2, MMP-9, MMP-14, and furin were also up-regulated. B, the precursor (P), intermediate (I), and matured (active) forms (M) of the MMPs and furin are indicated by arrows. A splice variant (SV) of Furin is also shown. β-Actin is used as the loading control. C, immunohistochemistry of lung tissues from UG-KO mice injected with B16F10 cells using antibodies against MMP-2, MMP-9, and MMP-14 (right panels). Anti-rabbit Alexafluor 594 (red) was used as a secondary antibody. H&E-stained tissue sections of same regions are shown at the left panels. H&E, hemotoxylin and eosin.