FIGURE 5.

Intestinal PMK-1 is required for survival on Y. pestis. Kaplan-Meier survival analysis of C. elegans strains challenged with Y. pestis KIM5 following RNAi knockdown of pmk-1 in the intestine (A), muscle (B), and hypodermis (C). Survival assays were performed as described under “Materials and Methods,” and animal survival was monitored every 12–24 h. Each plot represents the combined data of two or more experiments and a minimum of 90 animals (supplemental Table 2). Log rank analysis confirmed a significant decrease in survival following RNAi knockdown of pmk-1 in wild-type strain N2 (p < 0.0001) and strain VP303 (p < 0.0001) when compared with control RNAi treatment.