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. 2010 Feb 1;285(14):10862–10869. doi: 10.1074/jbc.M109.073171

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Maspin directed into the classical secretory pathway is not released into the extracellular milieu. A, comparison of the N terminus of HA/maspin and maspin. Boxed residues comprise the HA signal peptide. B, COS cells were transfected with maspin (upper panel) and HA/maspin (lower panel) DNA. Forty-eight hours after transfection, cells were used in a pulse-chase experiment as described under “Experimental Procedures.” COS cells transfected without DNA (mock) were included as a negative control.