FIGURE 6.

CUL7 overexpression in JEG-3 cells increased expression of ZEB1 and Slug, two transcriptional repressors of E-cadherin. A, the mRNA levels of ZEB1, Slug, Snail, Twist, SIP1, E-cadherin, CUL7, and GAPDH in stable CUL7-expressing JEG-3 cells and the mock-transfected cells (EV) were determined by RT-PCR. B, immunoblots show protein levels of ZEB1 and Slug were significantly higher in CUL7-expressing JEG-3 cells. WB, Western blot. C and D, siRNA interference of ZEB1 or Slug partially restored E-cadherin expression in JEG-3/CUL7 cells. The RT-PCR (C) and immunoblot (D) results shown were representatives of three independent experiments. Neg, negative controls.