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. 2010 Jan 24;285(14):10880–10889. doi: 10.1074/jbc.M109.081489

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Gene trap disruption of the Sgpl1 gene causes S1P lyase deficiency. A, sphingolipid metabolic pathway and linkage to triacylglycerol and phosphatidylethanolamine synthesis. B, appearance and life span of Sgpl1^−/−, Sgpl1^+/−, and Sgpl1^+/+ mice. C, RT-qPCR of Sgpl1 mRNA using RNA of various tissues from Sgpl1^−/− (gray bars) and Sgpl1^+/+ (open bars) mice. Gapdh was used as a reference gene for normalization. SI, small intestine. Data represent mean values ± S.E. D, top panel: Western blot of S1P lyase protein in various tissues from Sgpl1^−/− and Sgpl1^+/+ mice. Bottom panel, same blot probed with antibody to β-actin as a loading control. E, S1P lyase enzyme activity in extracts of various tissues from Sgpl1^−/− (gray bars) and Sgpl1^+/+ (open bars) mice. Data represent mean values ± S.E. of representative results from three independent experiments.