FIGURE 10.

Specific [³H]5HT uptake (A), abolition of amphetamine-stimulated [³H]5HT release (B), and marked reduction in the affinity of [³H]imipramine (C), resulting from the truncation of the SERT N terminus. A, uptake of [³H]5HT was determined in HEK293 cells transiently expressing wild type SERT and ^Δ64SERT as outlined in the legend to Fig. 1. B, PCA-induced release was determined as in Fig. 2, and the concentration-response curves generated as in Fig. 3. C, saturation of [³H]imipramine binding was performed with membranes (20 μg) prepared from HEK293 cells transiently expressing wild type SERT and ^Δ64SERT as outlined in the legends to Figs. 7 and 8.