FIGURE 9.

Expression of ^A64SERT and ^TACSERT in comparison with wild type SERT. The schematic representation illustrates the nature of the constructs employed in our study. The star indicates the position of Thr⁸¹. HEK293 cells were transiently transfected with cDNA encoding wild type or the ^Δ64SERT truncation mutant tagged with CFP (A and B) or the ^TACSERT (C). The cell surface expression of CFP-tagged transporters (A and B) was visualized by confocal microscopy by sequentially capturing images for CFP (green) and trypan blue (red; to delineate the cell surface). Overlays (in yellow) highlight the cell surface expression of the transporters. C, expression of ^TACSERT was visualized by staining fixed, nonpermeabilized cells with the M1 antibody directed against the N-terminal FLAG epitope followed by a secondary Alexa488-conjugated goat anti-mouse antibody using a Zeiss LSM510 confocal microscope (left image; the scale bar indicates 10 μm) as outlined under “Experimental Procedures”; the cell surface is shown in a bright field image (right image).