FIGURE 5.

S527A mutation of KCNQ2 channel abolished the inhibition of M currents by MβCD/Chol and PDBu. A–D, current inhibition induced by 150 μm MβCD/Chol (A and C) or 1 μm PDBu (B and D) were measured in HEK cells transfected with the wild-type KCNQ2 (A and B) or the mutant KCNQ2 (S527A) (C and D). The currents were elicited by 1-s steps to −60 mV from a holding potential of −20 mV at 5-s intervals as shown in the inset. E, effects of point mutation on the current inhibition of KCNQ2 channels induced by MβCD/Chol or PDBu. Values are expressed as means ± S.E. *, p < 0.01 compared with wild-type KCNQ2 channels.