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. Author manuscript; available in PMC: 2010 Apr 19.
Published in final edited form as: Nat Genet. 2007 May 27;39(6):797–802. doi: 10.1038/ng2051

Figure 1.

Figure 1

Steady-state levels of spontaneous RecB-dependent SOS induction demonstrate the presence of spontaneous DSBs and/or DSEs in a small cell subpopulation. (a–c) Representative flow cytometry histograms. (a) Positive control, GFP+ lexA51(Def) cells, and negative controls, lexA3(Ind−) and ΔrecA control cells. (b) Wild-type cells after exposure to ultraviolet C light. (c) Steady-state levels of SOS induction in wild-type, recA, recB and recB recA cells (full genotypes of all strains are given in Supplementary Methods and Supplementary Table 1). Horizontal bars represent the GFP+ gate. For b, ~ 0.9% of these cells are spontaneously green (see c,d), but the y-axis scale hides this shoulder to the main peak. For c, the recA and recB recA curves are both shown in gray, as the lines superimpose. (d) Quantification of steady-state levels of green wild-type cells (within the GFP+ gate) (mean ± s.e.m. of 18, 15, 7 and 4 experiments for wild-type, recB, recA and recB recA cells, respectively). In all figures (except where specified), sample groups with stars indicate statistical significance among all other starred sample groups using single-factor ANOVA and Tukey test, P < 0.01. Additionally, in Figure 1d, all strains are significantly different from all others (P < 0.01), except for recA and recB recA, which are not.