Table 3.
A. | B. | |||||
---|---|---|---|---|---|---|
Cell type | BAEC | MC3T3 | BAEC | MC3T3 | ||
Substrate | <10° | <25 | <10° | <25° | <10° | <10° |
Glass | 17 | 41 | 39 | 64 | – | – |
50 nm NP coating | 30 | 56 | 58 | 84 | +68% | +49% |
100 nm NP coating | 29 | 57 | 52 | 77 | +70% | +33% |
300 nm NP coating | 26 | 52 | 55 | 78 | +52% | +41% |
A. Percent of F-actin fibers exhibiting alignment within 10° and 25° of the long axis of the cell. Both BAEC and MC3T3 cells were cultured for a period of 24 h before fixing and staining. Unmodified glass, 50,100 and 300 nm silica NP-modified glass surfaces were tested. B. Percent increase in the number of F-actin fibers with alignment within 10° compared to glass surfaces.