Skip to main content
. 2010 Apr;10(4):751–762. doi: 10.1111/j.1600-6143.2010.03018.x

Figure 6.

Figure 6

Chimeras induced through Treg treatment demonstrate partial central and peripheral deletion of donor-reactive T cells late after BMT. (A) Percentages of Vβ11+ and Vβ5+ (but not Vβ8+) CD4+ splenocytes (SPL) were significantly reduced in Treg-treated mice (red bars, n = 7) in comparison to naïve B6 mice (gray bars, n = 5; p = 0.0003 for Vβ11+, p = 0.0309 for Vβ5+) and in comparison to BMT recipients without Tregs (black bars, n = 6; p = 0.0026 for Vβ11+, p = 0.0128 for Vβ5+; 25 weeks after BMT). (B) CD8+ splenocytes (SPL) were also significantly deleted in Treg-treated chimeras in comparison to naïve B6 controls, indirectly indicating central deletion (as mature superantigen-reactive CD8 cells are not deleted extrathymically) (p = 0.0002 for Vβ11+, p = 0.0141 for Vβ5+). (C) Percentages of Vβ11+ and Vβ5+ (but not Vβ8+) single-positive CD4+ thymocytes (THY) were significantly reduced in Treg-treated chimeras (red bars, n = 3) in comparison to naïve B6 mice (gray bars, n = 3; p = 0.0330 for Vβ11+, p = 0.0187 for Vβ5+) and in comparison to BMT recipients without Tregs (black bars, n = 7; p = 0.0102 for Vβ11+, p = 0.0151 for Vβ5+; 22 weeks after BMT). Deletion was assessed by multicolor flow cytometry in selected mice. Gray bars denote naïve B6 controls, white bars denote naïve Balb/c controls. p values are shown for comparison between groups (two-sided Student's t-test), error bars indicate standard deviation.