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. Author manuscript; available in PMC: 2010 Apr 19.
Published in final edited form as: Nature. 2008 Jun 11;454(7203):523–527. doi: 10.1038/nature07106

Figure 2. RIG-I-specific HCV RNA PAMP recognition and signaling.

Figure 2

a–e, Induction of the IFN-β promoter in cells co-transfected with 1 µg (20-30pmol) of tRNA control or the indicated HCV RNA species. a, Huh7.5 cells, lacking functional RIG-I, were cotransfected with a plasmid encoding vector alone or RIG-I. b, Promoter signaling in wild-type (wt) and RIG-I−/−, c, MDA5−/−, d, MyD88−/− or e, TRIF−/− mouse embryo fibroblasts. Asterisk indicate a significant difference (P<0.01) from tRNA control. f, FRET analysis of Cy3-labeled poly-U/UC RNA (Cy3-PUC) interaction with YFP-RIG-I or YFP-DAI protein in co-transfected Huh7 cells. Panels show representative images of YFP, Cy3, merged fluorescence, and N FRET (corrected FRET). The color scale denotes N FRET levels. The bar graph at right shows the calculated values for RNA interaction with RIG-I or DAI. Control values are from the image area that has no colocalization signal. All RNAs contain 5’ppp.