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. Author manuscript; available in PMC: 2010 Apr 19.
Published in final edited form as: Cell Death Differ. 2009 May 22;16(10):1323–1331. doi: 10.1038/cdd.2009.55

Figure 3. Both internal calcium stores and cell membrane calcium channels like the CRAC channels are important for the engulfment of apoptotic cells.

Figure 3

Figure 3

a) LR73 cells were incubated with labeled apoptotic thymocytes in the presence or absence of the indicated drugs and scored in the engulfment assay. Drugs shown were added at the same time as apoptotic thymocytes to minimize gross effects on the phagocytes. This is a representative of three independent experiments.

b) ORAI 1, 2 and 3 transcript levels in NIH3T3 cells transfected with siRNA specific for the genes indicated on the x-axis as measured by real-time PCR.

c) NIH3T3 that were transfected with the indicated siRNA were incubated with labeled apoptotic thymocytes and analyzed for engulfment. b&c are representative of two independent experiments.

Significance is indicated as follows: p>0.05 – (ns), 0.01–0.05 – (*), 0.001–0.01 – (**), <0.001 – (***).